Journal: Biomedical Reports
Article Title: Downregulation of sialyl-transferases and their role in malignant meningioma cells
doi: 10.3892/br.2025.2078
Figure Lengend Snippet: Suppression of sialylation does not affect the proliferation of meningioma cells. HKBMM cells were treated with various concentrations of 3Fax (0, 15.6, 31.2, 62.5, 125.0, 250.0, 500.0 µM) for 5 days. (A) Cytotoxic effect of 3Fax was assessed using the WST-8 viability. (B) Histogram plots and mean fluorescence intensity from lectin flow cytometry showed decreased expression of α2,3-linked MAL II and α2,6-linked SNA lectin sialic acids in 3Fax-treated cells (500 µM) compared with DMSO-treated control cells. (C and D) Cell proliferation and survival were assessed using the (C) WST-8 viability and (D) colony formation assays. Values are expressed as the mean ± standard deviation from three independent experiments. *** P≤0.001 and **** P≤0.0001 vs. control. MAL II, Maackia amurensis lectin II; SNA, Sambucus nigra lectin.
Article Snippet: The expression of sialic acids using Maackia amurensis lectin II (MAL II) and Sambucus nigra lectin (SNA; cat. no. PK-4000; Vector Laboratories Inc.) staining was performed on both treated and control cells, and the results were analyzed using an Attune TM NxT Acoustic Focusing Cytometer (Thermo Fisher Scientific, Inc.).
Techniques: Fluorescence, Flow Cytometry, Expressing, Control, Standard Deviation